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Published Workflows | puva | W1 - Pre-processing | Single-end

Galaxy Workflow ' W1 - Pre-processing | Single-end'

Annotation: Pre-processing of single-end reads

StepAnnotation
Step 1: Input dataset
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Reads in Fastq format
Step 2: FASTQ Groomer
Output dataset 'output' from step 1
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Convert forward reads to fastqsanger encoding
Step 3: FastQC:Read QC
Output dataset 'output_file' from step 2
QC reads - raw
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Quality control of forward reads
Step 4: FASTQ positional and quality trimming
Single-end
Output dataset 'output_file' from step 2
Trimming/filtering based on sequence quality and length
Step 5: FastQC:Read QC
Output dataset 'trimmed1' from step 4
QC reads - processed
select at runtime
Quality control of filtered reads